HYDRODEX cyclodextrin phases are made of cyclic oligosaccharides consisting of seven (β-cyclodextrin) or eight (γ-cyclodextrin) glucose units bonded through α-1,4-linkages. Regioselective derivatization of the hydroxyl groups leads to phases with varying enantioselectivity, which are well suited for chiral analyses. Due to the high melting point of the HYDRODEX phases they are diluted with polysiloxanes. Important advantage of cyclodextrin phases - many compounds can be analyzed without derivatization (however, for certain substances enantioselectivity can be favorably influenced by formation of derivatives). A large number of separations have been achieved, however, it is not possible to make a general prediction, which phase could solve a given separation task. Even for compounds with small structural differences or within homologous series the enantiodifferentiation can be quite different.

Column-Name	Phase	Tmax	Recommended Application
Hydrodex β-PM	Heptakis-(2,6-di-O-methyl-3-O-pentyl)-β-cyclodextrin	230‒250°C	hydroxycarboxylic acid esters, alcohols, diols, oleins, lactones, acetals
Hydrodex β-3P	Heptakis-(2,6-di-O-methyl-3-O-pentyl)-β-cyclodextrin	230‒250°C	terpenes, dienes, allenes, terpene alcohols, 1,2-epoxyalkanes, carboxylic acids (esters), hydroxycarboxylic acid esters, pharmaceuticals, pesticides
Hydrodex β-6TBDM	Heptakis-(2,3-di-O-methyl-6-O-tert-butyldimethylsilyl)-β-cyclodextrin	230‒250°C	γ-lactones, cyclopentanones, terpenes, esters, tartrates
Hydrodex β-6TBDE	Heptakis-(2,3-di-O-ethyl-6-O-tert-butyldimethylsilyl)-β-cyclodextrin	230‒250°C	essential oils
Hydrodex β-TBDAc	Heptakis-(2,3-di-O-acetyl-6-O-tert-butyldimethylsilyl)-β-cyclodextrin	220‒240°C	alcohols, esters, ketones, aldehydes, δ-lactones
Hydrodex γ-TBDAc	Octakis-(2,3-di-O-acetyl-6-O-tert-butyldimethylsilyl)-γ-cyclodextrin	220‒240°C	cyclic ketones, aromatic ketones, oxiranes, aromatic esters, aromatic amides
Hydrodex γ-DiMOM	Octakis-(2,3-di-O-methoxymethyl-6-O-tert-butyldimethylsilyl)-γ-cyclodextrin	220‒240°C	ketones, terpenes, cyclic ethers, alcohols, amines