SRT-C
SRT®-C SEC phase, developed based on innovative surface coating technology, comprises of uniform, hydrophilic, and neutral nanometer thick film chemically bonded to high purity and mechanically stabilized silica. SRT®-C, uses lay-down monolayer on porous silica as oppose to SRT of stand-up monolayer (as shown below), offers ideal phase chemistry for hydrophobic samples like insulin, membrane protein monoclonal antibodies derivatized with polymer branches (polypeptide or PEG) separation.
Filter Options
| Phase | SRT®-C SEC-150 | SRT®-C SEC-300 | SRT®-C SEC-500 |
| Material | Neutral, hydrophilic film bonded silica | ||
| Particle size | 5 µm | ||
| Pore size | ~ 150 Å | ~ 300 Å | ~ 500 Å |
| Protein MW range (native) | 500 - 150,000 | 5,000 - 1,250,000 | 15,000 - 5,000,000 |
| pH stability | 2 - 8.5 (pH 8.5-9.5 can be tolerated temporarily.) | ||
| Backpressure (for a 7.8×300 mm) | ~ 700 psi | ~ 700 psi | ~ 700 psi |
| Maximum backpressure | ~ 4,500 psi | ~ 3,500 psi | ~ 3,000 psi |
| Salt concentration range | 20 mM - 2.0 M | ||
| Maximum temperature | ~ 80 °C | ||
| Mobile phase compatibility | Aqueous and organic |
- Ideal for separation and analysis of hydrophobic proteins, and monoclonal antibodies derivatized with polymer branches such as polypeptide or PEG
- Particle size of 5 µm
- Selection of pore size: 100, 150, 300, 500, 1,000, and 2,000 Å
- High separation efficiency and resolution
- High capacity
- High lot-to-lot reproducibility
- High protein recovery with intact biological activity
- Negligible non-specific interactions
