TSKgel® Anion Exchange HPLC Columns

Tosoh

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TSKgel® Anion Exchange HPLC Columns

Tosoh

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Ion exchange chromatography separates molecules based on differences in the net charge of the molecules. In anion exchange, the analyte is negatively charged (anion), while the chromatographic material is positively charged (cation). Ion exchange is commonly used for protein purification, but it may be used for purification of oligonucleotides, peptides, or other charged molecules. For interaction to occur, the protein of interest must have a charge opposite to that of the functional group of the sorbent particle. Because the interaction is ionic, binding must take place under low ionic strength conditions. Elution is achieved by increasing the ionic strength of the mobile phase to reduce ionic attractions, or by changing the pH of the mobile phase to alter the ionization state of the protein. TSKgel columns are highly efficient for sample purification with excellent recovery. Anion- and cation-exchangers are available on porous polymer-based and silica-based particles. Proteins and nucleic acids can be analyzed faster on a TSKgel non-porous resin column, although STAT and NPR columns have lower capacity than large-pore-size-polymer particles. TSKgel BioAssist columns are constructed from PEEK (polyether ether ketone).
All Products for TSKgel® Anion Exchange HPLC Columns
Particle Size
10um 13um 20um
5um 2.5um 7um
Phase
ammonium ion quaternary DEAE polyamine
Length
7.5cm 15cm 3.5cm
2.5cm 1cm 5cm
10cm 25cm 2cm
0.5cm
Column ID
7.5mm 21.5mm 8mm
10mm 6mm 2mm
4.6mm 20mm 5mm
3mm
Part Number Part Name Particle Size Phase Length Column ID Qty
818257 TSKgel(R) SuperQ-5PW HPLC Column 10um ammonium ion quaternary 7.5cm 7.5mm
818387 TSKgel(R) SuperQ-5PW HPLC Column 13um ammonium ion quaternary 15cm 21.5mm
818386 TSKgel(R) SuperQ-5PW HPLC Column 10um ammonium ion quaternary 7.5cm 8mm
816092 TSKgel(R) DEAE-5PW Guardgel Kit 20um DEAE 3.5cm 10mm
818388 TSKgel(R) SuperQ-5PW Guardgel Kit 20um ammonium ion quaternary 2.5cm 6mm
842154 TSKgel(R) DEAE-SW Guardgel Kit 5um DEAE 1cm 2mm
819685 TSKgel(R) BioAssist(R) Q HPLC Column 10um polyamine 5cm 4.6mm
821410 TSKgel(R) BioAssist(R) Q HPLC Column 13um polyamine 10cm 10mm
807168 TSKgel(R) DEAE-SW Columns 5um DEAE 25cm 4.6mm
818761 TSKgel(R) DEAE-SW Columns 5um DEAE 25cm 2mm
807163 TSKgel(R) DEAE-SW Columns 10um DEAE 7.5cm 7.5mm
818757 TSKgel(R) DEAE-5PW HPLC Column 10um DEAE 7.5cm 2mm
807574 TSKgel(R) DEAE-5PW HPLC Column 13um DEAE 15cm 21.5mm
807164 TSKgel(R) DEAE-5PW HPLC Column 10um DEAE 7.5cm 7.5mm
808806 TSKgel(R) DEAE-5PW Guards and Guardgel Kits 20um DEAE 1cm 8mm
814016 TSKgel(R) DEAE-5PW HPLC Column 13um DEAE 15cm 20mm
813061 TSKgel(R) DEAE-5PW HPLC Column 10um DEAE 5cm 5mm
808802 TSKgel(R) DEAE-5PW HPLC Column 10um DEAE 7.5cm 8mm
814466 TSKgel(R) DEAE-5PW Guards and Guardgel Kits 13um DEAE 2cm 20mm
807210 TSKgel(R) DEAE-5PW Guardgel Kit 20um DEAE 2.5cm 6mm
813075 TSKgel(R) DEAE-NPR HPLC Column 2.5um DEAE 3.5cm 4.6mm
817088 TSKgel(R) DNA-NPR Guard Column 5um DEAE 0.5cm 4.6mm
807648 TSKgel(R) DEAE-SW Guardgel Kit 20um DEAE 2.5cm 6mm
818249 TSKgel(R) DNA-NPR HPLC Column 2.5um DEAE 7.5cm 4.6mm
818253 TSKgel(R) DEAE-NPR Guard Column 2.5um DEAE 0.5cm 4.6mm
821962 TSKgel(R) DNA-STAT HPLC Column 5um ammonium ion quaternary 10cm 4.6mm
807646 TSKgel(R) QAE-2SW Guardgel Kit 5um ammonium ion quaternary 2.5cm 6mm
807166 TSKgel(R) QAE-2SW HPLC Column 5um ammonium ion quaternary 25cm 4.6mm
821960 TSKgel(R) Q-STAT HPLC Column 10um ammonium ion quaternary 3.5cm 3mm
821961 TSKgel(R) Q-STAT HPLC Column 7um ammonium ion quaternary 10cm 4.6mm