Tosoh Bioscience has been a leader in the SEC analysis of monoclonal antibodies and other protein-based biotherapeutics since the start of the biotechnology revolution. First introduced in 1978 as the TSKgel® SW-type column line, the particle size of high efficiency TSKgel SW-type columns has steadily decreased from 10 to 5, to 4 and now 3 micron, while maintaining high pore volume and physical stability. The latest innovations in TSKgel column technology are focused on improving the quality of the purity analysis of therapeutic monoclonal antibody solutions and the determination of dimer and aggregate content. New TSKgel SW-type size exclusion columns and their mAb-related applications TSKgel SuperSW mAb HR for high resolution analysis of mAb monomer and dimer TSKgel SuperSW mAb HTP for high throughput analysis of mAb monomer and dimer (UHPLC compatible) TSKgel UltraSW Aggregate for the analysis of mAb aggregates In addition to the targeted benefits in resolution, throughput and aggregate analysis, the new TSKgel columns provide improvements in lot-to-lot variation and column life time, lower non-specific adsorption, and superior aggregate recovery. As shown in the figure, TSKgel SW mAb columns produce a shallow calibration curve centered around the molecular mass of a typical monoclonal antibody (~150,000 Da). As expected, since the columns differ only in particle size, the calibration curves for the TSKgel SuperSW mAb HR and the current industry standard TSKgel G3000SWxl columns overlap over the entire molecular weight range, while the linear portion of the calibration curve for the TSKgel UltraSW Aggregate column extends to a molecular mass of a few million dalton, which, in addition to the three micron particle size, benefits the separation of dimers and higher order aggregates from the monomer.