TSKgel® Gel Filtration Columns to Analyze mAb Purity and Aggregates

Tosoh

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TSKgel® Gel Filtration Columns to Analyze mAb Purity and Aggregates

Tosoh

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Tosoh Bioscience has been a leader in the SEC analysis of monoclonal antibodies and other protein-based biotherapeutics since the start of the biotechnology revolution. First introduced in 1978 as the TSKgel® SW-type column line, the particle size of high efficiency TSKgel SW-type columns has steadily decreased from 10 to 5, to 4 and now 3 micron, while maintaining high pore volume and physical stability. The latest innovations in TSKgel column technology are focused on improving the quality of the purity analysis of therapeutic monoclonal antibody solutions and the determination of dimer and aggregate content. New TSKgel SW-type size exclusion columns and their mAb-related applications TSKgel SuperSW mAb HR for high resolution analysis of mAb monomer and dimer TSKgel SuperSW mAb HTP for high throughput analysis of mAb monomer and dimer (UHPLC compatible) TSKgel UltraSW Aggregate for the analysis of mAb aggregates In addition to the targeted benefits in resolution, throughput and aggregate analysis, the new TSKgel columns provide improvements in lot-to-lot variation and column life time, lower non-specific adsorption, and superior aggregate recovery. As shown in the figure, TSKgel SW mAb columns produce a shallow calibration curve centered around the molecular mass of a typical monoclonal antibody (~150,000 Da). As expected, since the columns differ only in particle size, the calibration curves for the TSKgel SuperSW mAb HR and the current industry standard TSKgel G3000SWxl columns overlap over the entire molecular weight range, while the linear portion of the calibration curve for the TSKgel UltraSW Aggregate column extends to a molecular mass of a few million dalton, which, in addition to the three micron particle size, benefits the separation of dimers and higher order aggregates from the monomer.
All Products for TSKgel® Gel Filtration Columns to Analyze mAb Purity and Aggregates
Particle Size
4um 7um 3um
5um
Phase
DIOL
Length
30m 4cm 15cm
2cm 30cm
Column ID
7.8mm 6mm 4.6mm
3mm
Part Number Part Name Particle Size Phase Length Column ID Qty
822854 TSKgel(R) SuperSW mAb HR HPLC Column 4um DIOL 30m 7.8mm
822857 TSKgel(R) SuperSW mAb HR Guard Column 4um DIOL 4cm 6mm
822855 TSKgel(R) SuperSW mAb HTP HPLC Column 4um DIOL 15cm 4.6mm
822858 TSKgel(R) SuperSW mAb HTP Guard Column 4um DIOL 2cm 3mm
808543 TSKgel(R) SWXL Type Guard Column 7um DIOL 4cm 6mm
822856 TSKgel(R) UltraSW Aggregate HPLC Column 3um DIOL 30cm 7.8mm
822859 TSKgel(R) UltraSW Aggregate Guard Column 3um DIOL 4cm 6mm
808541 TSKgel(R) G3000SWXL HPLC Column 5um DIOL 30cm 7.8mm