Affinity chromatography allows purification of biomolecules on the basis of biological function or three-dimensional structure. The molecule to be purified is specifically and reversibly adsorbed by a complementary binding ligand immobilized on a matrix. The natural specificities of the interacting molecules offer high selectivities that can greatly reduce the time needed to purify the molecule. High-efficiency resin-based TSKgel affinity columns separate or purify many enzymes and other proteins.