Accucore C8 HPLC columns offer lower hydrophobic retention than columns packed with longer alkyl chain length material, such as C18, and are therefore recommended for analytes with medium hydrophobicity or when a less hydrophobic phase provides optimum retention. The low levels of secondary interactions demonstrated in the phase characterization are the result of excellent bonded phase coverage and allow users of Accucore C8 HPLC columns to benefit from excellent peak shapes.